anti atp5b rabbit polyclonal antibody  (Aviva Systems)

Journal: Frontiers in Oncology

Article Title: CHCHD4 Regulates Intracellular Oxygenation and Perinuclear Distribution of Mitochondria

doi: 10.3389/fonc.2017.00071

Figure Lengend Snippet: Elevated CHCHD4 expression causes localized intracellular hypoxia within the perinuclear region and constitutive hypoxia-inducible factor activation . (A) Images of immunostaining of U2OS cells (control) and CHCHD4 wild-type (WT)-expressing cells (clones WT.cl1 and WT.cl3) incubated with pimonidazole for 2 h. Cells were immunostained for pimonidazole (green) and ATP5B (mitochondria, red). Nuclei stained with DAPI (blue). Scale bar = 40 µm. (B) Graph shows expression of BNIP3 and CA9 analyzed by QPCR using total RNA isolated from cells described in (A) . Mean ± SD. * p < 0.05. (C) Graph shows the perinuclear proportion of mitochondrial fluorescence per cell, analyzed from cells described in (A) , after treatment without (−) or with (+) sodium azide (azide, 5 mM) for 16 h (10 fields of view per condition). n = 3; *** p < 0.001, * p < 0.05.

Article Snippet: The mouse monoclonal β-actin and rabbit polyclonal ATP5B antibodies were purchased from Abcam.

Techniques: Expressing, Activation Assay, Immunostaining, Clone Assay, Incubation, Staining, Isolation, Fluorescence